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PUBLICATIONS
Here you can find published examples of how researchers like you use the SPRimager®II array system and the SPR100 "FT-SPR" detector.

SPRimager®II
Protein-Nucleic Acid interactions

  • Assembly of viral capsid proteins on specific RNA sequences
    BH Garcia II, RM Goodman (2008): Use of surface plasmon resonance imaging to study viral RNA:protein interactions. J. Virological Meth. 147:18-25

  • Detecting Protein Biomarkers at Picomolar Concentration
    Y Li, HJ Lee, RM Corn (2007): Detection of Protein Biomarkers Using RNA Aptamer Microarrays and Enzymatically Amplified Surface Plasmon Resonance Imaging Analytical Chemistry 79:1082-8

  • RNA aptamer microarrays and protein–aptamer interactions
    Y Li, HJ Lee, RM Corn (2006): Fabrication and characterization of RNA aptamer microarrays for the study of protein–aptamer interactions with SPR imaging. Nucleic Acids Res. 34:6416-24

  • Binding of bacterial transcription factors to DNA promoters
    EA Smith, MG Erickson, AT Ulijasz, B Weisblum, RM Corn (2003): Surface plasmon resonance imaging of transcription factor proteins: interactions of bacterial response regulators with DNA arrays on gold films. Langmuir 19:1486-1492
     
  • Binding of eukaryotic transcription factors to wild type and mutant promoters. Compares SPR imaging and electrophoresis gel shift assays
    M Kyo, T Yamamoto, H Motohashi, T Kamiya, T Kuroita, T Tanaka, JD Engel, B Kawakami, M Yamamoto (2003): Evaluation of MafG interaction with Maf recognition element arrays by surface plasmon resonance imaging technique. Genes Cells 2:153-64
     
  • Specific binding of SSB to single stranded but not double stranded DNA
    JM Brockman, AG Frutos, RM Corn (1999) A multi-step chemical modification procedure to create DNA arrays on gold surfaces for the study of protein-DNA interactions with surface plasmon resonance imaging". J. Am. Chem. Soc. 121:8044-8051

    Related GWC Application Notes
    Monitoring Binding of SSB to ssDNA
    Monitoring DNA-DNA interactions

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Protein- Protein interactions

  • Interactions between Insulin and Insulin-degrading enzyme
    G Grasso, AI Bush, R D’Agata, E Rizzarelli & G Spoto (2008): Enzyme solid-state support assays: a surface plasmon resonance and mass spectrometry coupled study of immobilized insulin degrading enzyme Eur Biophys J 38:407-414
     
  • Binding affinity for the same calcium-dependent protein-protein interaction measured on the SPRimager®II and on a much more expensive instrument. As expected, the measured affinity is higher on the SPRimager®II
    L Subramanian, TM Walker, JC Takach, AS Polans (2006): An improved surface plasmon resonance method for determining the molecular basis of the calcium-dependent interactions of ALG-2. Calcium Binding Proteins 1: 54-61
     
  • Fabrication of His-tagged fusion protein arrays
    GJ Wegner, HJ Lee, G Marriott, RM Corn (2003) Fabrication of histidine-tagged fusion protein arrays for surface plasmon resonance imaging studies of protein-protein and protein-DNA interactions. Analytical Chemistry 75:4740-4746

    Related GWC Application Notes
    Preparation of a functional biotin surface on SpotReady chips
    Protein Immobilization

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Protein-Carbohydrate interactions

  • Binding of lectins to glycan arrays
    M Dhayal, DM Ratner (2009): XPS and SPR analysis of glycoarray surface density. Langmuir 25:2181-7
     
  • Binding of lectins to monosaccharide arrays
    EA Smith, WD Thomas, LL Kiessling, RM Corn (2003): Surface plasmon resonance imaging studies of protein-carbohydrate interactions. J. Am. Chem. Soc. 125: 6140-6148
     
  • Binding of Shiga-like toxin to oligosaccharide arrays; calculation of IC50 values for two synthetic inhibitors
    V Kanda, P Kitov, DR Bundle, MT McDermott (2005): Surface plasmon resonance imaging measurements of the inhibition of Shiga-like toxin by Synthetic Multivalent Inhibitors. Analytical Chemistry 77:7497-7504
     
  • Binding of Ricinus communis agglutinin (RCA) lectin to galactose conjugates
    R D’ Agata, G Grasso, G Iacono, G Spoto, G Vecchio (2006): Lectin recognition of a new SOD mimic bio-conjugate studied with surface plasmon resonance imaging. Org. Biomol. Chem. 4:610-612
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Antibody–Antigen interactions

  • MALDI-Mass Spectrometry Analysis of Biomarkers Captured on an Antibody Array
    D Nedelkov (2007): Development of Surface Plasmon Resonance Mass Spectrometry Array Platform. Anal Chem 79:5987-90
     
  • Epitope Analysis to Determine Compatible Antibody Pairs for Capture and Detection
    V Kodoyianni (2008): Versatile, Label-Free Microarray Analysis. Genetic Eng. News 28(5):42-43
     
  • Use of antibody arrays for multiplex detection of low molecular weight protein biomarkers
    HJ Lee, D Nedelkov, RM Corn (2006): Surface Plasmon Resonance Imaging Measurements of Antibody Arrays for the Multiplexed Detection of Low Molecular Weight Protein Biomarkers. Analytical Chemistry. 78:6504-6510
     
  • Affinities of antibody-peptide interactions measured by exposing peptide arrays to antibody
    GJ Wegner, HJ Lee, RM Corn (2002): Characterization and optimization of peptide arrays for the study of epitope-antibody interactions using surface plasmon resonance imaging. Analytical Chemistry. 74:5161-5168
     
  • Using antibody arrays to detect antigens in crude cell lysates
    M Kyo, K Usui-Aoki, H Koga (2005): Label-free detection of proteins in a crude cell lysate with antibody arrays by surface plasmon resonance imaging technique. Analytical Chemistry 77:7115-7121
     
  • Fabrication of antigen arrays to study antibody-antigen interactions
    V Kanda, JK Kariuki, DJ Harrison, MT McDermott (2004): Label-free reading of microarray-based immunoassays with surface plasmon resonance imaging. Analytical Chemistry 76:7257-7262

    Related GWC Application Notes
    Monitoring Antibody-Antigen interactions
    Antibody immobilization
    Measuring Antibody-Antigen Affinities

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Nucleic acid analysis

  • MicroRNA analysis
    A Wark, HJ Lee, RM Corn (2008): Multiplexed Detection Methods for Profiling microRNA Expression in Biological Samples. Angewandte Chemie 47:644-52

    S Fang, HJ Lee, AW Wark, RM Corn (2006): Attomole Microarray Detection of MicroRNAs by Nanoparticle-Amplified SPR Imaging Measurements of Surface Polyadenylation Reactions. J. Am. Chem. Soc. 128:14044-46

  • DNA and RNA hybridization to oligonucleotide arrays
    BP Nelson, TE Grimsrud, MR Liles, RM Goodman, RM Corn (2001): Surface plasmon resonance imaging measurements of DNA and RNA hybridization adsorption onto DNA microarrays. Analytical Chemistry 73:1-7

    BP Nelson, MR Liles, K Frederick, RM Corn, RM Goodman (2002): Label-free detection of 16S ribosomal RNA hybridization on reusable DNA arrays using surface plasmon resonance imaging. Environmental Microbiology 4:735-743

    HJ Lee, TT Goodrich, RM Corn (2001): SPR imaging measurements of 1D and 2D DNA microarrays in PDMS microfluidic channels on gold thin films. Analytical chemistry 73: 5525-5531
     
  • Induced hairpin formation and detection on arrays
    EA Smith, M Kyo, H Kumasawa, K Nakatani, I Saito RM Corn (2002): Chemically induced hairpin formation in DNA monolayers. J. Am. Chem. Soc. 124, 6810-6811

    K Nakatani, S Hagihara, Y Goto, A Kobori, M Hagihara, G Hayashi, M Kyo, M Nomura M Mishima , C Kojima (2005): Small-molecule ligand induces nucleotide flipping in (CAG)n trinucleotide repeats. Nature Chemical Biology 1:39-43
     
  • Detection of point mutations; SPR signal enhanced with nanospheres
    A Okumura, Y Sato, M Kyo, H Kawaguchi (2005): Point mutation detection with the sandwich method employing hydrogel nanospheres by the surface plasmon resonance imaging technique. Analytical Biochemistry 339:328-37

    H Kawaguchi, Y Sato, A Okumura, M Kyo (2005): Enhancement of sensitivity and selectivity in surface plasmon resonance detection of a DNA point mutation by polymeric microspheres. e-Polymers #050
     
  • Detection of 1fM DNA target using on-chip amplifcation
    TT Goodrich, HJ Lee, RM Corn (2004)
    Enzymatically amplified surface plasmon resonance imaging method using RNase H and RNA microarrays for the ultrasensitive detection of nucleic acids. Analytical Chemistry 76:6173-6178

    TT Goodrich, HJ Lee, RM Corn (2004): Direct detection of genomic DNA by enzymatically amplified SPR Imaging measurements of RNA microarrays. J. Am. Chem. Soc. 126:4086-4087

    S Fang, HJ Lee, AW Wark, HM Kim, RM Corn (2005): Determination of Ribonuclease H surface enzyme kinetics by surface plasmon resonance imaging and surface plasmon fluorescence spectroscopy. Analytical Chemistry 77:6528-6534
     
  • Monitoring binding of antibiotics to RNA arrays
    Y Nishimura, H Adachi, M Kyo, S Murakami, S Hattori, K Ajito (2005): A proof of the specificity of kanamycin-ribosomal RNA interaction with designed synthetic analogs and the antibacterial activity. Bioorg. Med. Chem. Lett. 15:2159-2162

    Related GWC Application Notes
    Monitoring DNA-DNA interactions
    Amplifast: a highly sensitive DNA detection method
    Monitoring Binding of SSB to ssDNA

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Making arrays

  • Antibody Array Methods
    CF Grant (2009): Development and Characterization of Interfacial Chemistry for Biomolecule Immobilization in Surface Plasmon Resonance (SPR) Imaging Studies. PhD Thesis, University of Alberta
     
  • Photopatterning
    JM Brockman, AG Frutos, RM Corn (1999): A multi-step chemical modification procedure to create DNA arrays on gold surfaces for the study of protein-DNA interactions with surface plasmon resonance Imaging. J. Am. Chem. Soc. 121:8044-8051
     
  • Line Arrays using PDMS
    HJ Lee, TT Goodrich, RM Corn (2001): SPR imaging measurements of 1D and 2D DNA microarrays in PDMS microfluidic channels on gold thin films. Analytical chemistry 73: 5525-5531
     
  • DNA Arrays
    Y Chen, A Nguyen, L Niu, RM Corn (2009): Fabrication of DNA Microarrays with Poly(l-glutamic acid) Monolayers on Gold Substrates for SPR Imaging Measurements. Langmuir 25: 5054–5060
     
  • EA Smith, MJ Wanat, Y Cheng, SVP Barreira, AG Frutos, RM Corn (2001): Formation, spectroscopic characterization and application of sulfhydryl-terminated alkanethiol monolayers for the chemical attachment of DNA onto gold surfaces. Langmuir 17: 2502-2507
     
  • Small molecule arrays
    N Kanoh, M Kyo, K Inamori, A Ando, A Asami, A Nakao, H Osada (2006): SPR imaging of photo-cross-linked small-molecule arrays on gold. Analytical Chemistry 78: 2226 -2230

    EA Smith, WD Thomas, LL Kiessling, RM Corn (2003): Surface plasmon resonance imaging studies of protein-carbohydrate interactions. J. Am. Chem. Soc. 125: 6140-6148

    Related GWC Application Notes
    How to prepare an array on SpotReady chips
    Preparing a functional streptavidin surface

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Kinetics analysis

  • Kinetic measurements of S protein–S peptide interactions using peptide arrays
    GJ Wegner, AW Wark, HJ Lee, E Codner, T Saeki, S Feng RM Corn (2004): Real time SPR imaging measurements for the multiplexed determination of protein adsorption /desorption kinetics and surface enzymatic reactions on peptide microarrays. Analytical Chemistry 76:5677-5684
     
  • Kinetic analysis of adsorption and desorption of RCA lectin on a glycoconjugate surface
    R D’ Agata, G Grasso, G Iacono, G Spoto, G Vecchio (2006): Lectin recognition of a new SOD mimic bioconjugate studied with surface plasmon resonance imaging. Org. Biomol. Chem.4:610-612
     
  • Kinetic analysis of RNaseH degradation of RNA probe arrays
    S Fang, HJ Lee, AW Wark, HM Kim, RM Corn (2005) Determination of Ribonuclease H surface enzyme kinetics by surface plasmon resonance imaging and surface plasmon fluorescence spectroscopy. Analytical Chemistry 77:6528-6534
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Whole cell characterization

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Monitoring peptide modifications

  • Monitoring peptide phoshorylation on peptide arrays
    K Inamori, M Kyo, Y Nishiya, Y Inoue, T Sonoda, E Kinoshita, T Koike, Y Katayama (2005): Detection and quantification of on-chip phosphorylated peptides by surface plasmon resonance imaging techniques using a phosphate capture molecule. Analytical Chemistry 77:3979-3985
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Carbon-on-Gold

  • Aldehyde Surface for Immobilizing Probes with Free Amines
    MR Lockett, MR Shortreed, LM Smith (2008): Aldehyde-Terminated Amorphous Carbon Substrates for the Fabrication of Biomolecule Arrays. Langmuir 24:9198–9203 

  • Original Description of this Robust Label-Free Surface
    MR Lockett, SC Weibel, MF Phillips, MR Shortreed, B Sun, RM Corn, RJ Hamers, F Cerrina, LM Smith (2008): Carbon-on-Metal Films for Surface Plasmon Resonance Detection of DNA Arrays. J. Am. Chem. Soc. 130:8611–8613
     
  • Analysis of protein array interactions
    HJ Lee, Y Yan, G Marriott, RM Corn (2005): Quantitative functional analysis of protein complexes on surfaces J. Physiology (London) 563:61-71

  • Chip fabrication and analysis by SPR imaging
    GJ Wegner, HJ Lee, RM Corn (2004): Surface plasmon resonance imaging measurements of DNA, RNA, and protein interactions to biomolecular arrays. Protein Microarray Technology, Wiley, ISBN: 3-527-30597-1, pp 107-129
     
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Reviews

  • Enzyme-Enhanced Assays for the SPRimager®II
    HJ Lee, AW Wark, RM Corn (2006): Creating advanced multifunctional biosensors with surface enzymatic transformations. Langmuir 22:5241-5250 

  • SPR instruments reviewed
    R Mukhopadhyay (2005): Surface plasmon resonance instruments diversify. Analytical Chemistry Aug 1
     
  • Analysis of protein array interactions
    HJ Lee, Y Yan, G Marriott, RM Corn (2005): Quantitative functional analysis of protein complexes on surfaces J. Physiology (London) 563:61-71

  • Chip fabrication and analysis by SPR imaging
    GJ Wegner, HJ Lee, RM Corn (2004): Surface plasmon resonance imaging measurements of DNA, RNA, and protein interactions to biomolecular arrays. Protein Microarray Technology, Wiley, ISBN: 3-527-30597-1, pp 107-129
     
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FT-SPR Applications
Original Description of the Method

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Recent applications of FT-SPR

  • S Boddohi, CE Killingsworth, MJ Kipper (2008)
    Polyelectrolyte Multilayer Assembly as a Function of pH and Ionic Strength Using the Polysaccharides Chitosan and Heparin Biomacromolecules 9:2021–2028

  • J Tang, RS Signarvic, WF DeGrado, F Gai (2007)
    Role of Helix Nucleation in the Kinetics of Binding of Mastoparan X to Phospholipid Bilayers Biochemistry 46:13856-13863

  • C Rhodes, S Franzen, J-P Maria, M Losego, DN Leonard, B Laughlin, G Duscher, S Weibel (2006)
    Surface plasmon resonance in conducting metal oxides. J. Appl. Physics 1000:54905

  • G Arena, A Contino, R D’Agata, C Sgarlata, G Spoto (2005)
    Ordered anchored cavities at work: a new and rapid SPR-based method for the detection of trace amounts of Cs+. New J. Chem. 11:1393-1395
     
  • G Grasso, R D’Agata, E Rizzarelli, G Spoto, L D’Andrea, C Pedone, A Picardi, A Romanelli, M Fragai, KJ Yeo (2005)
    Activity of anchored human matrix metalloproteinase-1 catalytic domain on Au(111) surfaces monitored by ESI-MS+ J. Mass Spectrom. 40:1565-1571
     
  • G Arena, A Contino, E Longo, C Sgarlata, G Spoto, V Zito (2004)
    Self-assembling, patterning and SPRimaging of a 1,3 alternate bis (dipyridyl)calix{4}arene derivative-Cu2+ complex immobilized on to Au(111) surfaces. Chem. Comm. 1812-1813

    Related GWC Application Notes
    The chemistries used for array fabrication and analysis developed for the SPRimager®II platform are readily ported to FT-SPR analysis on SPRchip™ substrates. SpotReady™ chips are not suitable for the SPR100 instrument.

    How to prepare the SPRchip™ for FT-SPR analysis.

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SPR Imager®II
FT-SPR