S. Rosa Rd
Madison, WI 53719
GWC’s SPRimager®II is designed
for analysis of molecular interactions in an array format. The
entire array is exposed to the same analyte in the 1-cm flow
cell, eliminating the channel-to-channel variability inherent
in multichannel systems, and allowing for simultaneous capture
of data for all probes on the array. You can view an example
of how interactions on arrays are monitored by watching this
The SPRimager®II uses Surface
Plasmon Resonance imaging (SPRi) to detect molecules as
they bind to probes on the surface of the array. SPRi works
without the use of labels such as fluorescent tags. Since labelling
molecules involves modifying them chemically, there is a risk
that the function may be impacted by the label, a particular
problem for proteomics research. Therefore label-free systems
such as the SPRimager®II are the preferred approach for
functional analysis of protein interactions.
SPRimager®II also lets you view the array images as you
collect them, and plots the progress of interactions at the
same time. This greatly accelerates methods development, letting
you keep an eye on experimental progress throughout the assay
period. There is no need to worry about quenching of fluorescent
labels when there aren’t any.
If you have multiple research interests, or should your experimental
needs change, you will appreciate the broad capabilities of
the SPRimager®II. Since SPRi can detect molecules regardless
of chemical composition, this system is as versatile as your
imagination: from antibodies to antibiotics, cells to cytokines,
ligands to lectins, oligonucleotides to oligosaccharides, toxins
to transcription factors, proteins to peptides, and all the
way from aptamers to zymogens, the SPRimager®II can handle
your future needs as well as your current ones.
don’t forget there’s more to proteomics than pairwise
interactions. With the SPRimager®II you can monitor multiple
sequential binding events, and analyze activities such as
ligand-dependent binding and competitive binding. The publications
list provides examples of the diversity of successful
research projects completed with this system.
The user-friendly SPRimager®II system puts you in the driver’s
seat, letting you select the best experimental design for your
particular project. You can choose any of GWC’s
application methods, select one from the growing body of
or develop your own protocols. And customers can always contact
For SPR imaging, you can make arrays on
chip substrates, using either a spotting robot or, for SpotReady™,
simple manual pipetting with the equipment you already own.
And all of GWC’s array substrates are manufactured dextran-free,
so you don’t have to worry about entangled polymers
impeding access of target molecules to the probes on your
All SPRimager®II systems include the pump, flow cell, computer
and software, ready for immediate use. You can have this system
up and running in your lab for less than you might expect, and
you don’t need to invest major resources learning to use
the system. A short introduction during installation is all
you need to get started with your experiments.
- Imaging capability for simultaneous capture of data
across the entire imaged area. High-sensitivity near-IR
CCD camera with high throughput collection lens delivers
high quality image data.
- Real-time data—images and graphical data may
be displayed in real time and saved for later analysis.
- Analyte delivery pump included with every system.
Collect data under continuous flow or stop-flow conditions.
- Polarization control for collection of s-polarized
light responses. Enables conversion to absolute reflectivity
values for direct comparison between experiments.
No need to settle for arbitrary relative units of
- Adjustable incident light angle range from 40°
to 70° allows for maximum flexibility in measuring
a wide variety of surface types. Enables collection
of SPR measurements in liquid and in air.
- Operating wavelength at 800nm provides optimum balance
of sensitivity and lateral resolution for SPR measurements
across the ~1-cm diameter circular flow cell. Custom
wavelengths are available, please contact
|For complete details
of the SPRimager®II system, including prices and specifications,
please contact your
with any questions or comments.